Interv Akut Kardiol. 2010;9(4):175-179

Cardiospecific markers during acute myocardial infarction - protein biochip utilization

Lucie Horáková¹, Radek Pudil¹, Miloš Tichý², Martina Ulrychová², Jan Vojáček¹

¹ I. interní klinika, FN a LF UK v Hradci Králové

² Ústav klinické biochemie a diagnostiky, FN a LF UK v Hradci Králové

The aim of the study was the monitoring of plasma levels of isoenzyme of creatine kinase (CKMB mass), myoglobin (MYO), BB isoenzyme

of glycogen phosphorylase (GPBB), heart-type fatty acid binding protein (H-FABP), carbonic anhydrase III (CA III), and cardiac troponin

I (cTnI) in patients with acute myocardial infarction during 5 days as well as screening of the sensitivity of the new technology of the

protein biochip microarray that was used to assess plasma levels of these markers (System Evidence Investigator™, Randox Laboratories

Ltd., Great Britain). The plasma level of H-FABP was markedly elevated in major part of patients in the first measurement (the peak of

plasma H-FABP 78,9 μg/l, ± 62,9 μg/l, vs. normal value 1,8 μg/l, ± 0,6 μg/l, P < 0,0001) and has the highest sensitivity for the determination

of the necrosis. The peak of plasma CKMB mass and cTnI was elevated throughout the time of the second measurement (the peak

of plasma CK MB mass 30,6 μg/l, ± 25,59 μg/l, vs. normal value, P < 0,0001 and cTnI 20,0 μg/l, ± 17,6 μg/l vs. normal value, P < 0,0001). The

plasma GPBB level was increased during the first measurement and the GPBB level slow decreased to the normal value. The CAIII plasma

level had the same course as the GPBB plasma level. In conclusion, we established new parameters (GPBB, H-FABP or CA III) that have

relation to the acute myocardial infarction and increase the specificity of standard markers of through the acute myocardial infarction

(and for MYO/CA III in the diagnosis of the re-infarction).

Keywords: myocardial infarction, markers of myocardial necrosis, protein´s biochip

Published: October 15, 2010  Show citation